Differentiation of Candida species obtained from nosocomial candidemia using RAPD-PCR technique

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Differentiation of Candida species obtained from nosocomial candidemia using RAPD-PCR technique.

Thirteen strains of the genus Candida were isolated from catheter, urine and surgical wounds from individual patients of the Santa Casa de Misericórdia, Belo Horizonte, MG, Brazil. Ten strains were characterized as Candida albicans, two as Candida glabrata, and one as Candida parapsilosis. Isolates were evaluated for molecular relatedness by random amplified polymorphic DNA technique using 15 p...

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Sequence-identification of Candida species isolated from candidemia

BACKGROUND Candida species are the most prevalent cause of invasive fungal infections such as candidemia. Candidemia is a lethal fungal infection among immunocompromised patients worldwide. Main pathogen is Candida albicans but a global shift in epidemiology toward non-albicans species have reported. Species identification is imperative for good management of candidemia as a fatal infection. Th...

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Identification of Candida species isolated from vulvovaginal candidiasis using PCR-RFLP

Vulvovaginal candidiasis (VVC) is a common disease among women worldwide, therefore, accurate and rapid diagnosis of causative agents based on molecular techniques utilizing amplification of target DNA is highly recomendad for epidemiological purposes and for effective treatment. The aim of this study was to identify clinically Candida species from VVC patients by restriction fragment length po...

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Species Identification and Strain Typing of Candida Isolates by PCR-RFLP and RAPD-PCR Analysis for Determining the Probable Sources of Nosocomial Infections

Background: Since the incidence of nosocomial Candida infections have been increasing in parallel with the raising in the number of patients involving in predisposing factors, determining the sources and the ways of acquisition of infection is necessary as an efficient strategy for controlling the diseases. The aim of this study is identification and strain typing of Candida strains isolated fr...

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Identification and antifungal susceptibility pattern of Candida species isolated from patients with nosocomial candiduria

Introduction: Nosocomial candiduria could be due to cystitis, pyelonephritis, or fungusball in the urinary tract system. Several reports have shown candidemia and upper urinarytract involvement as the complications of candiduria. The aim of this study was to assessnosocomial candiduria; identify Candida isolates and determine their drug susceptibilitypattern. Materials and Methods: Urine sample...

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ژورنال

عنوان ژورنال: Revista da Sociedade Brasileira de Medicina Tropical

سال: 2006

ISSN: 0037-8682

DOI: 10.1590/s0037-86822006000200008